Plant Tissue Culture Division
Laboratory Research
Sheel Biotech Ltd is having a biggest commercial laboratory in Northern & North
East of India, producing International Standard Tissue Culture plants of various
crops. 15000 sq feet state of art laboratory at Manesar is just 35 kms away from
International Airport of New Delhi.
The Lab is also equipped with fully controlled Greenhouses and Net houses to
hardned the plants for domestic and export markets.
Salient Features :-
Glimpses of Research Activities
Few Research Findings
TISSUE CULTURE BANANA CULTIVATION
Soil
All types of well drained soils with good water holding capacity are suitable for
Banana.
Spacing
Tall varieties 6 x 6 others 6 x 5
Land Preparation
Plough the soil to fine tilth. Make pits of 1 x 1 x 1' and fill 3/4th with soil:
Farm Yard Manure: Sand in the ratio of 1: 1: 1. The minimum Organic matter
should be about 5 Kgs. per pit.
Planting
Make slits in the polybag containing the plant, place it vertically in the pit
and fill the pit upto the level of soil in the polybag.
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Irrigation
Irrigate immediately after planting and follow this with once in 3-4 days. The
average consumption of water per plant is about 2-20 litres, depending upon the
age of the plant.
Fertigation
Five split doses are to be given for a good banana crop. Basic dose 30days after
planting.
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Ammonium Sulphate |
50gms./plant |
| N:P:K 19:19:19 |
100gms/plant |
| Muriate of potash |
125gms/plant |
| Urea |
50gms/plant |
| Total |
325gms/Plant |
| Top dressing Ammonium Sulphate |
165gms/plant |
| Single Super Phosphate |
150gms/plant |
Top dressing to be given on 2nd, 3rd, 4th and 6th month after planting.
Fertilizer can be applied at a radius of 8 inches from the base and should be
immediately watered after application. Dosage may be varied based on the soil
and recommendation.
Plant Protection
Tissue culture plants are disease free. To protect the plant from soil borne
infections, apply 3-4 gms of Phorate 10% granules per pit and mix with the soil
mixture seven days before planting. Irrigate the soil after application.
Desuckering
The suckers from the main plant should be removed at regular intervals by cutting
off the growing tip and piercing the base with a sharp instrument.
Flowering
Once the flowering is over, remove the male buds and trim the floral remnants to
avoid finger tip disease. Increase the watering frequency after flowering and
keep soil wet all the time.
Support
All banana plants require support in heavy wind areas. Grand Naine and Williams
require support under all conditions. Banana bunches should be covered with
perforated polythene bags or with dried leaves after all the hands emerge. This
prevents fruit diseases and the crop matures early.
Yield
The crop will be ready for harvesting in 10-12 months after planting. If managed
well, Dwarf Cavandish gives an average yield of 35 to 40MT/acre.
TISSUE CULTURE BAMBOO
Bamboos are giant, woody grasses which
produces long, naturally pre-finished Culms ("stems"). Because of its diverse
application and socioeconomic importance, the Bamboos are also called" Green
Gold".
Bamboo is the most diverse group of plants in the grass family and is
distinguished by a woody culm. It has a tropical and subtropical (cosmopolitan)
distribution, ranging from 46 N to 47 S latitude, reaching elevations as high as
4,000M in the Himalayas and parts of China. Bamboo is very adaptable, with some
species being deciduous and others evergreen
Propagation
Bamboos are conventionally propagated through seedlings and rooted cuttings.
Commercial varieties of bamboos take 12 to 150 years for flowering. In most of
the economically important species, there is an acute shortage of material.
Seeds are difficult to store and loose its viability fast. Cuttings are too
bulky for transportation and cannot be produced in large numbers.
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Considering the importance of producing and making available large number of
planting materials, the only alternative is in vitro micropropagation.
The advantages of Micropropagation are:
Elite clones can be identified and propagated.
Production of plantlets throughout the year
Planting materials can be produced in large numbers with less space.
In vitro produced plants establish and grow fast
Biomass production is much higher compared to seedling progenies.
Easy to transport over long distances.
Sheel Bio-Tech producing Bamboos in vitro techniques house technology as
collaboration with institutions in India and Abroad
